摘要:ScienceLondon第三方抗体验证数据库,来自抗体用户的反馈报告,为你提供最真实,最可靠的抗体选择参考!真正避免抗体带来的实验可重复性问题!His-Tag抗体验证-SL0011

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A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag (registered by EMD Biosciences). The tag was invented by Roche,?although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are available from Qiagen, Sigma, Thermo Scientific, GE Healthcare, Macherey-Nagel, Clontech, Bio-Rad, and others

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一抗名称: His-tag polyclonal antibody(STJ90106)100?l

供应商: St John's Laboratory Ltd

稀释比例: 1:1000

二抗名称: HRP goat anti-rabbit (provided by BPI)

内参抗体:Actin (provided by BPI)

抗体: STJ90106. His-tag antibody

验证方式: Western Blot

验证样本: HeLa cell line, Jurkat cell line

样本处理: When cells were cultured to 70%-80% plating density, lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer were added for 10 min water bath at 100?C.

产品信息:His-tag Antibody

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