摘要:ScienceLondon第三方抗体验证数据库,来自抗体用户的反馈报告,为你提供最真实,最可靠的抗体选择参考!真正避免抗体带来的实验可重复性问题!HA-Tag抗体验证-SL0009

[vc_row row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern" css_animation=""][vc_column][vc_tabs style="horizontal"][vc_tab title="抗体验证报告-PDF" tab_id="3a796117-c60d-10"][vc_column_text]STJ90107-HA-tag
[/vc_column_text][/vc_tab][vc_tab title="关于目标蛋白" tab_id="dd172f8e-f410-5"][vc_column_text]

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The HA tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity.

[/vc_column_text][/vc_tab][/vc_tabs][/vc_column][/vc_row][vc_row row_type="row" use_row_as_full_screen_section="no" type="full_width" angled_section="no" text_align="left" background_image_as_pattern="without_pattern" css_animation=""][vc_column][vc_separator type="normal" up="20" down="50"][vc_column_text]

一抗名称: HA-tag Polyclonal Antibody(STJ90107) 100?l

供应商: St John's Laboratory Ltd

稀释比例: 1:1000

二抗名称: HRP goat anti-rabbit (provided by BPI)

内参抗体: Actin (provided by BPI)

抗体: STJ90107. HA-tag Polyclonal Antibody

验证方法: Western Blot

验证样本: HeLa cell line, Jurkat cell line

样本处理: When cells were cultured to 70%-80% plating density, lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer were added for 10 min water bath at 100?C.

验证效果: Target band was found in the material cell lines.

产品信息:HA-Tag Antibody

[/vc_column_text][/vc_column][/vc_row][vc_row row_type="row" use_row_as_full_screen_section="no" type="grid" angled_section="no" text_align="left" background_image_as_pattern="without_pattern" css_animation=""][vc_column][vc_separator type="transparent" up="20" down="50"][button size="big_large" icon="" target="_self" hover_type="default" font_weight="900" text_align="center" text="查看更多抗体验证报告" link="www.london-science.com/validation"][vc_separator type="transparent" up="20" down="50"][/vc_column][/vc_row]